Наукові праці. Кафедра медичної біології

Permanent URI for this collectionhttps://repo.knmu.edu.ua/handle/123456789/316

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Assessment of efficiency of cryoprotectant mixtures containing various antioxidants
(2019) Babijchuk, L.; Мakashova, O.; Zubov, P.; Zubova, O.
The use of cord blood (CB) hematopoietic progenitor cells (HPCs) is firmly established in practical medicine as an effective method for treating diseases of various genesis. This has led to the development of protocols of low-temperature storage and the cryobanks network. DMSO at 7.5-10% concentrations is the most common cryoprotectant for cryopreservation of CB nucleated cells (NCs) including HPCs.
Association of one-carbon metabolism-related genes and ichthyosis vulgaris manifestation in Eastern Ukraine
(Nature Publishing Group, 2022-05) Fedota, Olena; Sadovnychenko, Iurii; Makukh, Halyna; Chorna, Lilia; Roshcheniuk, Larissa; Vorontsov, Vitalii; Ryzhko, Pavlo
The study is devoted to analysis of the effects of one-carbon metabolism-related genes on the IV clinical manifestation in FLG mutation carriers.
Atherogenesis predictors in patients with nonalcoholic fatty liver disease
(2015) Sytnyk, K.; Tverezovska, I.; Kurinna, Olena
Awareness of young maldivians (medical students) about β-thalassemia
(2019) Kalian, V.; Nauma, H.; Faziha, M.
Studies show that Maldives has one of the world’s highest thalassemia carrier rate (F.Waleed et al., 2016). Professor Calogero Vullo (Italy) in 1992 estimated that 1 out of every 5 to 6 person in Maldives is a carrier for β-thalassemia. Approximately 28 new β -thal cases are recorded annually. Poor awareness and not fully knowing the devastating consequences of the condition are main reasons for this high number of new cases (F. Waleed et al., 2016). The main primary prevention strategy is awareness with premarital screening and genetic counseling. However, the solution of this problem has ethical aspect. Gender, education level, age, being single, income level (Al-Farsi et al., 2014) and fear of stigmatization (Fahad et al., 2012) contribute to unwillingness to participate in premarital screening. Besides, religion plays a vital role in all decisions. In our opinion, medical education must help to raise people’s awareness. The purpose of this study was to assess the initial degree of awareness of young Maldivians (medical students about β -thalassemia disease and their personal attitude to solution of the problem existing in the country. The survey has been undertaken among Maldivian students that study in Kharkiv National Medical University (KNMU), Ukraine. Twenty-three students aged 19 to 29 years old participated in the survey (18 females and 5 males). The anonymous questionnaire had four groups of questions about: (i) personal data (place of living in Maldives, gender, age, education level, marriage status); (ii) if a respondent has been informed about β-thalassemia situation in Maldives and disease consequences; (iii) young Maldivians' awareness about premarital screening and The Thalassemia prevention law of Maldives; (iv) students' attitude to amniocentesis in Maldives and possibility to terminate pregnancy legally. According to obtained results, 48% of Maldivian students represent Male’ while 52% are from different atolls. Assessment of the degree of young Maldivians awareness about β-thalassemia disease shows that 30% of students do not know that Maldives has one of the world’s highest β-thal carrier rate and they are not informed about the disease devastating consequences. 100% of all respondents know where they can do premarital screening for carrier status. 35% of KNMU Maldivian students have undergone premarital screening. The main reasons why students did not undertake premarital screening are: 1) being single; 2) lack of time; 3) difficulty to access the services; 4) lack of knowledge about thalassemia. Majority of students (78%) have heard about The Thalassemia prevention law of Maldives, but only 48% understand meaning of this law. The study of young Maldivians’ personal attitude to solution of the problem existing in the country shows that 78% of students believe that amniocentesis should be allowed in Maldives, 17% of them think that it should not be allowed and 5% hesitate. To sum up, the study demonstrates that more than 50% of young KNMU Maldivians are not ready to accept some possible medical intervention to prevent birth of β-thal children. The main reasons of that are religion and culture. Our task for future is to conduct activities to raise awareness of young Maldivians who study at KNMU about β-thalassemia, because being doctors they may contribute to the solution of the tremendous problem posed by thalassemia in the country.
Awareness of young nigerians about malaria disease
(2017) Kalyan, V.; Khromenkova, Olga; Myronova, I.; Ukaogo, D.
Bos taurus як модельний об’єкт порушень репродукційної функції людини, пов’язаних з поліморфиними варіантами генів гормону росту та рецептору гормону росту
(2018) Лисенко, Н.Г.; Мітіогло, Л. В.; Понько, Л. П.
Comparative analysis of the role of domestic allergens in atopic dermatitis etiologi in children
(2015) Shmulich, V.; Uryvaeva, M.; Lupaltsova, Olha; Staruseva, V.; Rybka, O.; Shmulich, O.; Kryvenko, L.
Four hundred and thirty-eight children aged 4 to 18 suffering from atopic dermatitis in the sustained remission phase in the course of the disease were studied using the skin prick testing technique. Prevalence of children of pre-school and primary school age with the atopic dermatitis was established. Skin acariasis caused by Dermatophagoides farinae mites prevails which can be used to optimize specific immunotherapy of atopic of dermatitis in children. Children of the senior school age were the least sensitive to A. Daphnia magna. Suitability of the start elimination therapy was proved. The detected age and sex etiological features of (atopic dermatitis) AD in the large population of children related mostly to the prevailing role of domestic triggers suggest the urgent necessity to eliminate the allergens in the residential premises where children suffering from allergic diseases live, as well as in the places where healthy children live, since the long contact with the indoor allergens is potentially hazardous regarding sensitization formation.
Comparison of reproductive characteristics of women from Chernivtsi and Simferopol
(2018) Kozak, N.; Antsupova, V.; Mustafaeva, L.
Reproductive characteristics were calculated for the residents of Chernivtsi and the residents of Simferopol of post-productive age according to the data of the obstetric-gynecologic anamnesis. The average number of pregnancies and their outcomes for the representatives of these cities were count for two generations. Statistical differences between populations were found by the percentage of women who had medical abortions, miscarriages and stillbirths. Significant difference was found for the percentage of artificially interrupted pregnancies and the realized zygotes.
Development of soft and hard skills for PhD students of Medical University as a tool for the formation of highly qualified scientific and pedagogical staff
(2021) Myroshnychenko, Mykhailo; Kapustnik, Valeriy; Myasoedov, Valeriy
Ecologo-hygienic concept and preventive measures for the protection of water recreational areas of river Siverskyi Donets
(2015) Shcherban, M.; Kapustnik, Valeriy; Myasoyedov, V.; Lytvynenko, M.; Krivonis, K.; Vasenko, O.
Effect of Surface Modification of Sputtered Ta2O5 Magnetron Ceramic Coatings on the Functional Properties of Antigen-Presenting Cells In Vitro Tests
(2020) Yakovin, S.; Dudin, S.; Zykova, A.; Safonov, V.; Goltsev, A.; Dubrava, T.; Rassokha, I.
The effect of surface treatment of Ta2O5 nanostructured coatings by argon ions and electron beam on the functional potential of antigen-presenting cells of the monocyte-phagocytic system has been studied. The adhesive potential, indicators of phagocytic and metabolic activity of the studied cells depending on the surface properties of magnetron sputtered tantalum pentoxide coatings were analyzed. Electron irradiation process led to the stimulation of adhesive potential, phagocytic and metabolic activity of cells on the Ta2O5 coated surfaces. On the contrary, the surface treatment by argon ions significantly reduced the functional activity of the studied cells
Effects of 5 SNPs on daughters' milk performance traits produced by Ukrainian dairy sires
(2018) Fedota, O.; Lysenko, N.; Mitiohlo, L.; Ruban, S.
The aim of the study was to estimate the effects of the CAPN316, CAST282, GH L127V, GHR F279Y and A257G on milk yield, fat and protein yield and content on daughters produced by dairy sires. 24 dairy sires were 100% Holstein (14), 100% Brown Swiss (5) and Holstein mixed with another dairy breeds (5). Analyses included data on 32733 daughters for 2014-2017 years. Molecular genetic analysis was performed by PCR-RFLP. Statistical analysis revealed that population was on Hardy-Weinberg equilibrium. SNPs F279Y and A257G were not in linkage disequilibrium (r2 = 0.029, D’ = 0.074), because linkage of certain alleles was observed only in 7.4% of cases (D’). C-allele of SNP CAPN316 was strongly associated with protein content, despite C-allele of SNP CAST282 showed negative association with milk, fat and protein yield. Daughter milk performance traits (DMPTs) for SNP CAPN316 corresponded to CC>CG>GG pattern, except variations in milk and fat yield (kg). For SNP CAST282 Milk, fat and protein yield (kg) were significantly higher for CG than for CC genotype. C-allele for SNP L127V showed significant association and differences between genotypes with DYDs for fat yield (kg) – 20.45±5.53 (P<0.01) and content (%) – 0.13±0.06 (P<0.05), T-allele for SNP F279V was not significantly associated with any trait studied and A-allele for SNP A257G significantly associated with milk and fat yield (kg) for DMPTs/DYDs 1988.33±419.93/-269.69±89.92 (P<0.01) and 84.30±15.64/-8.33±3.87 (P<0.05). Results of this study reveal and support known advantages of SNPs studied in panels and selection programs.
Effects of CYP3A28 gene on productive traits and reproductive health of dairy and beef cattle
(2018) Fedota, O.; Lysenko, N.; Mitiohlo, L.; Babalian, V.; Tyzhnenko, T.; Mazniakov, S.; Valilshchikov, M.; Ruban, S.
Effects of Non-Allelic Interactions of O2 and SU2 Mutant Genes on Grain Biochemical Composition in Various Corn Inbreds
(2023-10-31) Tymchuk, Dmytro; Sadovnychenko, Iurii; Tymchuk, Natalia; Pasiuga, Olga
The use of combinations of non-allelic mutant genes of the maize endosperm structure creates opportunities for improving the quality of corn grain in comparison not only with forms of the common type but also with monogenic endospermic mutants. In this study, the effect of a combination of mutant genes O2 (Opaque-2) and SU2 (Sugary-2) according to the biochemical composition of the grain was studied. For the research, a series of inbreds - carriers of a combination of mutant genes O2SU2, inbreds - carriers of monogenic mutations O2 and SU2, as well as maize inbreds of the common type of two-year reproduction were used. In the experiments, the content of protein, starch, and oil and the main characteristics of their quality were studied. It was found that the inbred carriers of the O2SU2 combination are superior to the inbred carriers of monogenic mutations O2 and SU2 in terms of complex biochemical characteristics. In comparison with mutants O2 they were distinguished by an increased content of protein (by 12.3% on average), amylose in starch (by 38.9% on average), starch digestibility (by 24.4% on average), oil content (by 18.4% on average) and oleate content in oil (by 29.9% on average). In comparison with the carriers of SU2 mutation, they had a higher content of lysine and tryptophan in the total grain protein (on average, by 19.4% & 14.3%, respectively). The main characteristics of grain quality in carriers of a combination of mutant genes O2SU2 were characterized by quantitative variability, which can modify the effect of non–allelic interaction of mutant genes O2 and SU2. The obtained results indicate the effectiveness of using non-allelic interactions between the O2 and SU2 mutant genes to improve the quality of corn grain.
The Effects of Polymorphisms in One-carbon Metabolism Genes on Manifestation of Ichthyosis Vulgaris
(2021) Fedota, Olena; Sadovnychenko, Y.; Chorna, L.; Roshchenyuk, Larysa; Vorontsov, Vitalii; Ryzhko, Pavlo; Haiboniuk, I.; Belyaev, S.; Belozorov, I.; Makukh, H.
BACKGROUND: Ichthyosis vulgaris is the most common type of Mendelian disorders of cornification, caused by loss-of-function mutations in the gene encoding epidermal protein filaggrin (FLG), namely R501X and 2282del4. FLG 2282del4 mutation in heterozygotes is incompletely penetrant. Polymorphisms in one-carbon metabolism genes could be associated with clinical manifestation of ichthyosis vulgaris. AIM: The purpose of the present study was to analyze the effects of MTHFR, MTR, and MTRR polymorphisms in patients with ichthyosis vulgaris. METHODS: Thirty-one patients with ichthyosis vulgaris, 7 their FLG heterozygous relatives without symptoms of disorder, and 150 healthy controls were enrolled in the study. FLG null mutations — R501X (rs61816761) and 2282del4 (rs558269137) — and one-carbon metabolism gene polymorphisms — MTHFR C677T (rs1801133), MTHFR A1298C (rs1801131), MTR A2756G (rs1805087), and MTRR A66G (rs1801394) — were analyzed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. RESULTS: Among patients with ichthyosis, heterozygous for FLG 2282 del4 mutation, the distributions of genotypes for folate metabolism genes were: MTHFR C677T CC:CT:TT —29.4%:70.6%:0.0%; MTHFR A1298C AA:AC:CC — 52.9%:47.1%:0.0%; MTR A2756G AA:AG:GG — 70.3%:23.5%:5.9%; and MTRR A66G AA:AG:GG — 23.4%:52.9%:23.5%. The frequencies of MTR 2756AA and MTRR 66GG genotypes were 1.4–1.6 times higher in affected individuals heterozygous for 2282del4 than in patients with other FLG genotypes. In affected 2282del4 heterozygotes, the frequency of MTR 2756AA genotype was 1.6 times greater than in healthy controls (p < 0.01). The strongest association was found between MTHFR 677CT/MTHFR 1298AA/MTR 2756AA/MTRR 66AG genotype and ichthyosis — odds ratio (OR)=11.23 (95% confidence interval 2.51−50.21, p = 0.002). CONCLUSIONS: Various genotypes of one-carbon metabolism genes increase the risk of ichthyosis in heterozygotes for the FLG 2282del4 mutation (OR 2.80–11.23). The most probable predisposing genotype is 677CT/1298AA/2756AA+AG/66AG
Epidemiological aspect of global dracunculiasis eradication
(2018) Kalyan, V.; Myronova, I.; Younus Ahmed; Hana Aishath
Estimation of inbreeding depression by CAPN1, CAST, GH, GHR and CYP3A genes for quantitative and reproduction traits in seven Aberdeen-Angus lines
(2017) Fedota, O.; Ruban, S.; Lysenko, N.; Kolisnyk, A.; Goraichuk, I.; Tyzhnenko, T.
Experimental therapy of graft-versus-host disease by mesenchymal stromal cells grown on oxide nanocoatings
(2016) Goltsev, A.; Rassokha, I.; Dubrava, T.; Ostankova, L.; Ostankov, M.; Safonov, E.; Zykova, A.
Immune aggression to transplanted allogeneic bone marrow, i.e. the graft-versus-host disease (GVHD), could be decreased by the suppression of effector and/or activation of T- regulatory cells (Тreg). This task could be solved by co-transplantaiton of allogeneic bone marrow and mesenchymal stromal cells (MSCs). This study demonstrated the elevated immune modulating activity of MSCs by their culturing in vitro on Al2O3 oxide nanocoatings. Introduction of the cells to the animals with GVHD resulted in an increased content of Treg in the spleen of bone marrow recipients, reduced severity of the pathology, and higher survival of animals. The findings could be the basis for developing the new approaches to optimize the GVHD treatment methods involving the oxide nanocoating cultured MSCs.
Genetic studies of the MTHFR gene polymorphic variants within the framework of individual genetic safety
(2017) Fedota, O.; Roschenyuk, L.; Tyznenko, T.; Admakina, A.; Goraichuk, I.; Vorontsov, V.; Ryzhko, P.
Genetic Study of X-Linked Recessive Ichthyosis in Eastern Ukraine
(2021) Fedota, О.; Roshcheniuk, L.; Sadovnychenko, I.; Gontar, J.; Merenkova, I.; Vorontsov, V.; Ryzhko, P.
X-linked recessive ichthyosis (OMIM 308100) is a form of ichthyosis caused by abnormal keratinization and can result in disability, social maladaptation, and decreased quality of life for patients and their families. In most cases the disease is caused by a complete or partial deletion of the steroid sulfatase (STS) gene. This study estimated the prevalence of X-linked recessive ichthyosis, the inbreeding coefficient (or fixation index) FST, and the selection coefficient in individuals of eastern Ukraine (namely, Kharkiv oblast). The genealogical method was used to assess the genetic structure of families with a history of this disease. Fluorescent in situ hybridization (FISH) was carried out to detect the deletion of the STS gene in patients and their relatives. The prevalence of the disease in eastern Ukraine was 1.5 × 10–4 males, it ranged from 4.9 × 10–5 to 4.9 × 10–4 males in the districts, and from 2.2 × 10–4 males in the town of Krasnograd to 3.7 × 10–3 males in a village of Balakliia district. The burden of X-linked recessive ichthyosis was found to be positively correlated with the inbreeding coefficient FST in all the studied districts (r = 0.976). Over the past 10 years, the inbreeding coefficient FST increased 1.8 times and the prevalence of X-linked recessive ichthyosis increased 1.4–4.3 times in most districts of the region.