Reactive oxygen species generation by blood leucocytes of rats with polycystic ovary syndrome under the conditions of intermittent cold exposure

dc.contributor.authorZhulikova, Maryna
dc.contributor.authorMyroshnychenko, Mykhailo
dc.contributor.authorNakonechna, Oksana
dc.contributor.authorZhulikov, Oleh
dc.contributor.authorPustova, Nataliia
dc.contributor.authorBibichenko, Viktoriia
dc.contributor.authorLytvynenko, Olena
dc.contributor.authorKucheriavchenko, Maryna
dc.date.accessioned2023-11-16T09:03:10Z
dc.date.available2023-11-16T09:03:10Z
dc.date.issued2023
dc.description.abstractThe aim of this study was to determine the level of ROS production by blood leukocytes of rats with PCOS under the conditions of intermittent cold exposure. Materials and methods: In the study, 40 immature female rats of the WAG population at the age of 27 days with a body weight of 80-90 g were used. Five groups were formed (8 animals in each group). Group 1 was represented by intact rats that were not subjected to any manipulations. Group 2 was represented by rats that were injected subcutaneously with 0.2 ml of purified and sterilized olive oil daily for 25 days. Group 3 was represented by rats that were exposed to intermittent cold for 25 days. Group 4 was represented by rats that were modeled with PCOS. Group 5 was represented by rats, which were simulated PCOS against the background of intermittent cold exposure. ROS production was estimated in leukocytes isolated from rats of all groups by flow cytometry using the fluorescent probe of 2,7-dichlorodihydrofluorescein diacetate (H2DCFDA). Results: The experimental study revealed an intracellular excessive production of ROS by leukocytes in rats with polycystic ovary syndrome. The use of intermittent cold exposure normalized the production of reactive oxygen species by leukocytes in rats with polycystic ovary syndrome. Conclusions: The effectiveness of intermittent cold exposure, proven by the authors, allows recommending its use as one of the methods of prevention and treatment of the polycystic ovary syndrome.en_US
dc.identifier.citationReactive oxygen species generation by blood leucocytes of rats with polycystic ovary syndrome under the conditions of intermittent cold exposure / M. V. Zhulikova, M. S. Myroshnychenko, O. A. Nakonechna, O. O. Zhulikov, N.A. Pustova, V.O. Bibichenko, O. Yu. Lytvynenko, M. O. Kucheriavchenko // Wiadomosci Lekarskie Medical Advances. – 2023. – Vol. 76 (7). – P. 1670─1676.en_US
dc.identifier.urihttps://repo.knmu.edu.ua/handle/123456789/32889
dc.language.isoenen_US
dc.subjectleukocytesen_US
dc.subjectratsen_US
dc.subjectreactive oxygen speciesen_US
dc.subjectpolycystic ovary syndromeen_US
dc.subjectintermittent cold exposureen_US
dc.subjectoxidative stressen_US
dc.subject2023аen_US
dc.titleReactive oxygen species generation by blood leucocytes of rats with polycystic ovary syndrome under the conditions of intermittent cold exposureen_US
dc.typeArticleen_US

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