01. Кафедри
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Item Nerve Conduction and Neuromuscular Transmission in C57Bl/6 Mice with Genetically Determined Peripheral Neuropathy(2019-07) Govbakh, I.; Zavodovskiy, D.; Bulgakova, N.; Sokołowska, I.; Maznychenko, A.; Vasylenko, D.Charcot–Marie–Tooth disease is one of the most widespread forms of hereditary peripheral neuropathy in humans. C57Bl/6 mice are considered the most appropriate animal model for studies of this disease. We measured the conduction velocity in the sciatic nerve (NCV) and amplitude of the M-wave in mice of strains C57Bl/6 and C57Bl. It was found that the mean conduction velocity in the right and left sciatic nerves of C57Bl/6 mice was about 3.5-fold lower than that in C57Bl animals. In the former mice, the mean amplitude of compound nerve action potentials (CNAPs) and that of the M-wave in the m. gastrocnemius-soleus after stimulation of the sciatic nerve were 5- and 4-fold, respectively, lower, than those in the control (C57B1). Thus, the data obtained show that the genetically determined pathology of the peripheral nervous system caused by a mutation of the PMP22 gene results in dramatic negative shifts of the characteristics of conduction via the peripheral nerves and of neuromuscular transmission.Item Coordination of locomotor activity in transgenic C57Bl/6 mice with hereditary neuropathy(2019-09-30) Hovbakh I.,; Zavodovskiy, D.; Bulgakova, N.; Tsupykov, O.; Vasylenko, D.; Maznychenko, A.Locomotor activity of C57Bl/6 mice with hereditary motor and sensory neuropathy (HMSN; an animal model of Charcot–Marie–Tooth, CMT, disease) was investigated in males and females of two ages (15 and 20 weeks) using the balance beam test (inclined beam); such indices as time of traveling via the beam to the shelter and number of slippings of the hindlimbs from the beam were recorded. It was found that C57Bl/6 mice spent dramatically more time for traveling than control C57Bl mice with no neuropathy, and the number of erroneous movements (slippings of the hindlimbs) during traveling in mice with HMSN was many times greater than that in the controls. The deficiency of control of locomotion in C57Bl/6 animals was found to be sex- and age-dependent. Females of this strain moved significantly slower than males of the same age categories; both 20-week-old males and females with HMSN spent significantly more time for traveling the test distance than 15-week-old animals and demonstrated more motor failures. Thus, symptoms of HMSN are more pronounced in females (probably due to the specificity of the hormonal background in the latter), and the severity of pathology increases with age. The balance beam test appears acceptable for obtaining easily interpretable quantitative characteristics of the quality of locomotion control in experimental animal models of neuropathies.Item Epithelial-mesenchymal transformation as a mechanism of sclerosis development in the kidneys, ureters and bladder of fetuses and newborns from mothers, whose pregnancy was complicated by iron deficiency anemia and preeclampsia(2019) Sorokina, I; Danylchenko, S; Myroshnychenko, Mykhailo; Kapustnyk, N; Lytvynenko, OA comprehensive analysis of the immunohistochemical reactions revealed the sign of epithelial-mesenchymal transformation in the kidneys, ureters and bladder of fetuses and newborns, characterized by loss of the cells epithelial phenotype and acquisition of mesenchymal phenotype, which were more pronounced in the cases of their development in conditions of maternal preeclampsia and less pronounced in maternal iron deficiency anemia, increased from the fetus to the newborn and with the maternal pathology degree of severity enlargement. Epithelial-mesenchymal transformation, which takes an active part in the development of such irreversible process as sclerosis, in the urinary system organs of fetuses and newborns, which developed in conditions of maternal preeclampsia and iron deficiency anemia, must be given due attention that in future will help to find new methods of prevention and treatment.Item Доцільність використання й інформативність багатопрофільного тесту для виявлення наркотиків у сечі SNIPER® 10 на госпітальному етапі в дітей із гострою інфекційною патологією(2019) Міщенко, Вікторія Анатоліївна; Георгіянц, Маріне Акоіпвна; Корсунов, Володимир Анатоліїович; Пушкар, Михайло Борисович; Пороша, Наталія Станіславівна; Степанова, Ганна Аркадіївна; Мацак, Денис ЮрійовичItem Optimization of postoperative analgesia after adenoidectomy in children(2017) Georgiyants, Marine Akopivna; Pushkar, Mykhailo Borysovych; Vysotska, Olena Volodymyrivna; Porvan, Andrii PavlovychItem Optimization of current postoperative period after childrens’ adenotomy(2017) Georgiyants, Marine; Pushkar, Mykhailo; Vysotska, Olena; Porvan, AndriiItem Анатомо-фізіологічні особливості нервової системи у дітей різних вікових груп(2017) Георгіянц, Маріне Акопівна; Корсунов, Володимир Анатолійович; Раскова, Тетяна Юріївна; Одинець, Ігор Юрійович; Лисенко, Лідія Сергіївна; Пушкар, Михайло БорисовичItem Особливості анестезіологічного забезпечення хірургічних маніуляцій у дітей(2018) Корсунов, Володимир Анатолійович; Георгіянц, Маріне Акоіпвна; Раскова, Тетяна Юріївна; Одинець, Ігор Юрійович; Лисенко, Лідія Сергіївна; Пушкар, Михайло БорисовичItem Клінічна фізіологія та патологія водно-електролітного гомеостазу(2017) Георгіянц, Маріне Акопівна; Корсунов, Володимир Анатолійович; Одинець, Ігор Юрійович; Раскова, Тетяна Юріївна; Пушкар, Михайло Борисович; Лисенко, Лідія СергіївнаItem Анатомо-фізіологічні особливості терморегуляції дитячого організму(2017) Георгіянц, Маріне Акопівна; Корсунов, Володимир Анатолійович; Раскова, Тетяна Юріївна; Одинець, Ігор Юрійович; Лисенко, Лідія Сергіївна; Пушкар, Михайло Борисович